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| Знайдено в інших БД: | Журнали та продовжувані видання (1) | Реферативна база даних (20) |
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Загальна кількість знайдених документів : 7 Представлено документи з 1 до 7 |
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| 1. | 
Bilyk O. O. The frequency of human papilloma virus types 16, 18 in upper genital tract of women at high risk of developing ovarian cancer [Електронний ресурс] / O. O. Bilyk, N. T. Pande, T. Pejovic, L. G. Buchynska // Experimental oncology. - 2014. - Vol. 36, № 2. - С. 121-124. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_2_13 Aim - to investigate the incidence of human papilloma vims (HPV) types 16, 18 in upper genital tract of women considered at a high risk (HR) of developing epithelial ovarian cancer (EOC). Methods: HPV 16 and 18 E6 ORF specific semiquandtative PCRwas used to screen the incidence of HPV in 20 women at HR of developing EOC and 10 women with no ovarian disease (control). The HR subset of fallopian tubes and ovarian tissues showed greater positrvity for HPV E6 ORF (40 %) as compared to control (10 %) tissues. Of all the samples, two (10 %) were positive for HPV 16, two (10 %) were positive for HPV 18, and four (20 %) showed positivity for mixed HPV 16/18 infection. The presence of HPV E6 ORF was found both in the fallopian tubes and ovarian DNA from 6 (30 %) patients. In two cases (10 %) we detected HPV ORF only in the fallopian tube derived genomic DNA. Conclusion: it has been shown the presence of HPV in the upper genital tract in women at HR of developing EOC in close proximity of HPV susceptible tissue cervix. | ||
| 2. |
Chekhun S. V. Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells [Електронний ресурс] / S. V. Chekhun, N. Y. Lukyanova, Y. V. Shvets, A. P. Burlaka, L. G. Buchynska // Experimental oncology. - 2014. - Vol. 36, № 3. - С. 179-183. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_3_8 Aim - the aim of our study is to investigate the disorders of ferritin functioning in breast cancer (BC) cells of different molecular subtype. The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF- 10A, and 184A1. Ferritin heavy chains (FTH) expression was studied by immunohistochemical method. "Free iron" content and superoxide dismutase (SOD) activity were determined by means of EPR spectroscopy. Reactive oxygen species (ROS) level and peculiarities of microRNA expression in studied cell lines were evaluated using flow cytometry and PCR analysis, respectively. It has been demonstrated, that FTH expression directly correlates with proliferative activity of cells of both luminal (r = 0,51) and basal subtypes (r = 0,25), inversely correlates with expression of steroid hormones in cells of basal subtype (ER; r = -0,46; PR: r = -0,44) and does not depend on tumorigenic activity of both subtypes of studied cells (r = 0,12 and r = 0,9). Obtained data are the evidence, that cells of luminal subtype B (MCF-7 cell line) and basal subtype (MDA-MB-231 and MDA-MB-468 cell lines) with high proliferative activity contain the highest level of free iron (2,9 +- 0,19 x 1016 and 3,0 +- 0,22 x 1016), that can be consequence of intensive use of this element by cells, which actively divide and grow. Along with it, in cell of lines of basal subtype MDA-MB-231 and MDA-MB-468, high level of FTH (254 +- 2,3 and 270 +- 1,9) Is being detected in consequence of increase of level of free iron, ROS (11,3 +- 1,05 and 7,27 +- 0,26) and SOD (9,4 +- 0,24 and 8,5 +- 0,18) as well as decrease of expression of microRNA 200b. In contrast, cells oflumina) subtype B of MCF-7 line were distinguished by high expression of microRNA 200b and low ferritin level (125 +- 2,7). Conclusion: obtained data demonstrate that tumor cells, which are referred to different molecular subtypes, arc characterized by changes in system of support of balance of intercellular iron and certain associations of studied factors. | ||
| 3. |
Nesina I. P. The study of chromosomal instability in patients with endometrial cancer [Електронний ресурс] / I. P. Nesina, N. P. Iurchenko, S. V. Nespryad’ko, L. G. Buchynska // Experimental oncology. - 2014. - Vol. 36, № 3. - С. 202-206. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_3_12 Aim - study is devoted to evaluation of sensitivity of peripheral blood T-lymphocytes (PBL) of patients with endometrial cancer (EC) to genotoxic effect of bleomycin and detection of patients with hidden chromosomal instability. Methods: PBL of 24 EC patients (mean age 58,9 +- 2,9) and 10 healthy women-volunteers (mean age 55,7 +- 2,3) were subjected to cytogenetic analysis. Mean spontaneous level of chromosomal aberrations (CA) per 100 analyzed lymphocytes (CA/100) of healthy women has equaled 2,7 +- 0,6, i.e. has not exceeded maximal values of healthy population and was significantly lower (p << 0,05), than in PBL of EC patients (6,9 +- 0,6). After incubation of PBL with bleomycin, number of CA/100 significantly was increased both in control (11,5 +- 1,3) and in EC patients (21,9 +- 1,0). Spontaneous chromosomal instability has been observed in 41,7 %, increased sensitivity to bleomycin - in 54,2 % and hidden chromosomal instability in 37,5 % of patients with EC. It has been shown that level of specific damage of genome in EC patients has constituted 2 x 10-5, and after exposure with bleomycin, it was increased 4,5 times (9 x 10-5), that was significantly higher (p << 0,05) compared to control (8 x 10-6 and 1,0 x 10-5, respectively). Conclusion: these results have demonstrated, that PBL of most patients with EC are characterized by apparent genome alterations, which are manifested by increased number of spontaneous and induced chromosomal damages, hypersensitivity to mutagens and hidden chromosomal instability. | ||
| 4. |
Glushchenko N. M. Risk assessment of cancer of the female reproductive system [Електронний ресурс] / N. M. Glushchenko, I. P. Nesina, N. P. Iurchenko, L. A. Proskurnya, L. G. Buchynska // Experimental oncology. - 2014. - Vol. 36, № 3. - С. 207-211. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_3_13 Aim - to create an information resource concerning multifactorial oncological diseases of (he female reproductive system. A comprehensive search of the literature in the PubMcd and Ukrainian scientific sources published from 1995 to 2014 and the results of researches performed in R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine. Development environment of information resource "Multifactorial oncological disease" was Borland Delphi. The information content of web page concerning cancers of the female reproductive system was posted in the information resource "Multifactorial oncological disease". The assessment algorithm of genetic contribution to cancers of the female reproductive system and recurrent risk of cancer development in families have been described. These algorithms can be used in assessment of contribution of genetic and environmental factors in the development of malignant tumors. | ||
| 5. |
Iurchenko N. P. Comprehensive analysis of intratumoral lymphocytes and FOXP3 expression in tumor cells of endometrial cancer [Електронний ресурс] / N. P. Iurchenko, N. M. Glushchenko, L. G. Buchynska // Experimental oncology. - 2014. - Vol. 36, № 4. - С. 262-266. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_4_11 Aim - to study the tumor microenvironment (CD4+, CD8+ and FOXP3+ lymphocytes) and FOXP3 expression by tumor cells and correlation of studied parameters with clinical and morphological characteristics of endometrial adenocarcinomas. Tumor samples from 40 patients (mean age 56,9 +- 2,8) with endometrial cancer (EC), who did not receive special treatment before surgery (chemotherapy, radiation therapy and hormontherapy), were investigated. Morphological, immunohis-tochemical methods as well as methods of mathematical statistics were applied in the study. It has been determined that high quantity of FOXP3+ tumor cells and intratumoral CD4+ and CD8+ T-Iymphocytes along with the low content of FOXP3+-lymphocytes is typical for the endometrial adenocarcinomas of high differentiation grade (G1). In poorly differentiated (G3) EC an increase of number of FOXP3+-lymphocytes and decrease of CD4+ and CD8+ lymphocytes in lymphocytic infiltrate have been observed. Moreover, decrease of the content of FOXP3+ tumor ceils has been determined. In EC patients correlation between the following parameters has been detected: proliferative activity and deep invasion of tumor in myometrium (R = 0,74); depth of invasion correlated with the number of the FOXP3+ tumor cells (R = -0,63) and number of CD4+ and CD8+ lymphocytes (R = 0,68 and R = -0,55 respectively) in lymphocytic infiltrate. Thus, results of this study are the evidence of significance of the lymphocytic components of tumor microenvironment and content of FOXP3 expressing tumor cells in EC progression. Conclusion: quantitative changes of tumor microenvironment, such as number of CD4+, CD8+ and FOXP3+ lymphocytes and content of FOXP3+ tumor cells correlate with biological characteristics of EC. | ||
| 6. |
Buchynska L. G. FOXP3 gene promoter methylation in endometrial cancer cells [Електронний ресурс] / L. G. Buchynska, N. P. Iurchenko, N. P. Verko, K. A. Nekrasov, V. I. Kashuba // Experimental oncology. - 2015. - Vol. 37, № 4. - С. 246-249. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2015_37_4_4 Aim - to determine the methylation level of promoter region of the FOXP3 gene promoter depending on the heterogeneity of intracellular localization of its protein product in endometrial cancer (EC) cells and assess its relation to the clinical and morphological features of tumor. Samples of surgical material of 40 EC patients who have not received any specific treatment before the surgery, were studied. Real time methylation-specific PCR (MSP) as well as morphological and immunohis-tochemical methods were used in the study. Methylation of promoter region of the FOXP3 gene was determined in all EC cases, but variability of the methylation level in EC cells from 45,0 % to 85,0 % was observed. With tumor progression and in tumors with deep (<$Esymbol У~1 "/" 2>) invasion in myometrium, an increase of the methylation level of the FOXP3 and of cell number with cytoplasmic FOXP3 localization was observed. In EC patients the correlation between of methylation level of the FOXP3 gene and the number of FOXP3<^>+ | ||
| 7. |
Buchynska L. G. The study of mismatch repair in endometrial cancer patients with a family history of cancer [Електронний ресурс] / L. G. Buchynska, O. V. Brieieva, K. A. Nekrasov, S. V. Nespryadko // Experimental oncology. - 2015. - Vol. 37, № 4. - С. 272-276. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2015_37_4_8 Aim - to assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer. Morphological and immunohistochemical study was performed on tumor tissue samples of 49 EC patients. Microsatellite instability was determined using PCR with primers which flank microsatellite region BAT-26. A tendency to a decreased expression of both MSH2 and MLH1 markers in a group of EC patients with a family history of cancer as compared with a group without aggregation of cancer in family history was observed (labeling index - LI - was 36,1 +- 8,1 % and LI 20,7 +- 9,1 % versus LI 48,0 +- 5,8 % and 33,8 +- 5,8 %, respectively). It was determined that the number of EC patients with tumors deficient by expression of MMR markers was reliably higher in a group of patients with a family history of cancer than in a group of patients without aggregation of cancer in family history (p << 0,05). It was shown, that in a group of EC patients with a family history of cancer, MMR-proficient tumors were detected in 38,5 % of cases. Microsatellite instability was determined in 10,7 % of EC patients including one patient with aggregation of Lynch-associated tumors in family history. Conclusion: family history of cancer of EC patients is associated with malfunctioning of the MMR system as well as may be related to alternative molecular mechanisms. | ||
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